ms 200 microarray scanner Search Results


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(A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. <t>Microarray</t> experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.
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(A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. <t>Microarray</t> experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.
Microarray Scanner, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. <t>Microarray</t> experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.
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(A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. <t>Microarray</t> experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.
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(A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. <t>Microarray</t> experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.
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Agilent technologies microarray scanner g2565ba
(A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. <t>Microarray</t> experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.
Microarray Scanner G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies g2565ca microarray scanner system
(A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. <t>Microarray</t> experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.
G2565ca Microarray Scanner System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. Microarray experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.

Journal: PLoS ONE

Article Title: Striatal Molecular Signature of Subchronic Subthalamic Nucleus High Frequency Stimulation in Parkinsonian Rat

doi: 10.1371/journal.pone.0060447

Figure Lengend Snippet: (A) Genes specifically up-regulated by HFS, (B) opposite regulation of Pkrcd by HFS and DOPA and (C) common down-regulation of Sirt5 by HFS and DOPA. Microarray experiments were performed on total striatal RNA samples from 6-OHDA, HFS, DOPA and DOPA/HFS groups. For qPCR validation of gene expression, total striatal RNA samples from 3 groups were analyzed: control, 6-OHDA and HFS. For both microarray and qPCR, results were calculated for each sample relative to the expression of the endogenous reference gene: HPRT, and fold change vs the 6-OHDA group was determined using the 2 −ΔΔCt method. Values are presented as means ± SEM. *p<0.05 vs 6-OHDA values.

Article Snippet: For microarray experiments, amplification and fluorescent labeling of RNA was performed with the kit LIRLAK one color (Low Input RNA Linear Amplification Kit, Agilent) with starting amounts of 200 to 400 ng of total RNA. cRNAs were hybridized on Rat AGILENT 4×44 K microarrays and, after adequate washing, scanned with Agilent Microarray Scanner.

Techniques: Microarray, Expressing